Fecha/hora: 21/05/2024 02:53:04

Biblioteca(s): 	INIA Las Brujas.	
Fecha corriente: 	02/05/2023	
Fecha de la última actualización: 	02/05/2023	
Autoria: 	BARAIBAR, S.; IRIARTE, W.; DE LEON, W.; BONNECARRERE, V.	
Afiliación: 	SILVINA BARAIBAR PEDERSEN, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; WANDA IRIARTE GRECO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; WILMAR DAMIAN DE LEON CARRO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARIA VICTORIA BONNECARRERE MARTINEZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay.	
Título: 	Validation of SNP markers for selection of semi-dwarf and peduncle extrusion in barley.	
Año de publicación: 	2023	
Fuente/Imprenta: 	Molecular Breeding, 2023, Volume 43, Issue 4, Article 30. https://doi.org/10.1007/s11032-023-01371-7	
ISSN: 	1380-3743	
DOI: 	10.1007/s11032-023-01371-7	
Idioma: 	Inglés	
Notas: 	Article history: Received 16 May 2022; Accepted 16 March 2023; Published online 13 April 2023. -- Corresponding author: Bonnecarrère, V.; Instituto Nacional de Investigación Agropecuaria (INIA), Area Mejoramiento Genetico y Biotecnologia Vegetal, Estación Experimental INIA Las Brujas, Ruta 48 km 10, Canelones, Uruguay; email:vbonnecarrere@lb.inia.org.uy  -- FUNDING: This work was funded by the INIA (Instituto Nacional de Investigación Agropecuaria) Projects: Mejoramiento de cultivo de secano and Mejoramiento molecular de cultivos. --	
Contenido: 	This study aimed to validate the use of two SNP markers associated to a sdw1 allele identified previously in a short barley genotype (ND23049) with an adequate peduncle extrusion which reduces predisposition to fungal disease development. First, the GBS SNP were converted in a KASP marker but only one of them, named TP4712, correctly amplified all allelic variations and had a Mendelian segregation in a F2 population. To corroborate the association between TP4712 allele with plant height and peduncle extrusion, a total of 1221 genotypes were genotyped and evaluated for both traits. Out of the 1221 genotypes, 199 were F4 lines, 79 were a diverse panel, and 943 were two complete breeding cohorts of stage 1 yield trials. To corroborate the association between the sdw1 allele and the short plant height with adequate peduncle extrusion, contingency tables were created, grouping the 2427 data points into categories. Based on the contingency analysis, it was demonstrated that the higher proportion of short plants with adequate peduncle extrusion were present in genotypes carrying the SNP allele of ND23049 regardless the population and the sowing date. This study develops a marker-assisted selection tool to accelerate the introgression of favourable alleles for plant height and peduncle extrusion in adapted germplasm. © 2023, The Author(s), under exclusive licence to Springer Nature B.V.	
Palabras-Clave: 	ÁREA MEJORAMIENTO GENÉTICO Y BIOTECNOLOGÍA VEGETAL - INIA; Breeding population; Marker-assisted selection; Peduncle extrusion; Plant height; SISTEMA AGRÍCOLA-GANADERO - INIA; SNP marker.	
Categoría del tema: 	F30 Genética vegetal y fitomejoramiento	
Registro original: 	INIA Las Brujas (LB)	
Ejemplares: 	LB - (103412-1) - PXI - PC - DD - Molecular Breeding/2023 -  -  - DIS